Metabolite‐mediated catalyst conversion of PFK and PFP: a mechanism of enyme regulation in green plants

FEBS Letters

Wong, Joshua H.¹  Wötzel, Christiane¹  Cséke, Csaba¹  Soll, Jürgen¹  Buchanan, Bob B.¹  Balogh, Árpád¹ 

[1] Division of Molecular Plant Biology, Hilgard Hall, University of California, Berkeley, CA 94720, USA

关键词: FPLC;    Phosphofructokinase;    Metabolite-mediated catalyst conversion;    Pyrophosphate—D-fructose-6-phosphate 1-phosphotransferase;    Fru-6-P;    fructose-6-phosphate;    PPi;    inorganic pyrophosphate;    PFP;    pyrophosphate—D-fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.90);    PFK;    phosphofructokinase (EC 2.7.1.11);    Fru-1;    6-P2;    fructose 2;    6-bisphosphate;    Fru-2;    6-P2;    fructose 2;    6-biphosphate;    FPLC;    fast protein liquid chromatography;    Glu-1;    6-P2;    glucose 1;    6-bisphosphate;    UDPG;    UDP-glucose;   

DOI  :  10.1016/0014-5793(84)80335-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metabolites known to occur in the cytosol of photosynthetic leaf cells were found to mediate the reversible conversion of pyrophosphate—D-fructose-6-phosphate 1-phosphotransferase (PFP) to phosphofructokinase (PFK) in partially purified preparations from spinach leaves. Preincubation of PFP with fructose 2,6-bisphosphate, ATP or fructose 6-phosphate converted PFP to PFK. The reverse reaction (PFK → PFP) was promoted by UDP-glucose plus pyrophosphate. These conversions in catalytic capability were accompanied by changes in molecular mass and charge. The results are in accord with the view that the alterations in PFP and PFK activity, provisionally called ‘metabolite-mediated catalyst conversion’, represent a regulatory mechanism to direct left cytosolic carbon flux in either the biosynthetic or degradatory direction.

【 授权许可】

Unknown