A novel pyrophosphate fructose‐6‐phosphate 1‐phosphotransferase from carrot roots Relation to PFK from the same source

FEBS Letters

Wong, Joshua H.¹  Kang, T.¹  Buchanan, Bob B.¹ 

[1] Division of Molecular Plant Biology, Hilgard Hall, University of California, Berkeley, CA 94720, USA

关键词: Pyrophosphate fructose-6-phosphate;    1-Phosphotransferase;    Phosphofructokinase;    Enzyme property;    Enzyme comparison;    Fru-6-P;    fructose-6-phosphate;    Fru-1;    6-P2;    fructose-1;    6-bisphosphate;    PFK;    phosphofructokinase;    PFP;    pyrophosphate fructose-6-phosphate 1-phosphotransferase;    PPi;    pyrophosphate;    Pi;    orthophosphate;    Tricine;    (N-tris[hydroxymethyl]methylglycine);    FPLC;    fast protein liquid chromatography;   

DOI  :  10.1016/0014-5793(88)80521-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel type of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) has been purified from carrot roots. The enzyme differs from its counterpart studied from other sources in subunit composition, response to regulatory metabolites and kinetic parameters. The properties of the enzyme are in accord with its function in a tissue that stores substantial quantities of sucrose. In describing the carrot root PFP, we have systematically compared its properties to those of phosphofructokinase from the same tissue. The results support the conclusion that, despite noteworthy similarities, the two enzymes are independent proteins, unlikely to undergo interconversion.

【 授权许可】

Unknown