HMG‐like proteins of Physarum polycephalum: Association with the transcriptionally active chromatin

FEBS Letters

Czupryn, Marta¹  Toczko, Kazimierz¹ 

[1] Institute of Biochemistry, Warsaw University, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland

关键词: Physarum polycephalum;    High mobility group protein;    Transcriptionally active chromatin;    HMG;    high mobility group;    PMSF;    phenylmethylsulphonyl fluoride;   

DOI  :  10.1016/0014-5793(84)80313-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

HMG-like proteins were isolated from nuclei of the acellular slime mold Physarum polycephalum. These proteins represented 0.6 ± 0.2% of nuclear proteins and approx. 6% of DNA by weight. Polyacrylamide gel electrphoresis demonstrated the presence of 3 major proteins, designated HMG-1P, HMG-2P and HMG-14/17P. None of these proteins comigrated with any of the calf thymus HMGs. Physarum HMG-like proteins were found to be preferentially associated with transcriptionally active chromatin fraction, as well as being released from nuclei by light DNase I digestion. In contrast, the content of histone H1 was significantly lower in active than in inactive chromatin fraction.

【 授权许可】

Unknown