Stimulatory effect of the maize HMGa protein on reporter gene expression in maize protoplasts

FEBS Letters

Grasser, Klaus D.¹  Feix, Günter¹  Griess, Eike A.¹  Hetz, Winfried¹ 

[1] Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Schänzlestr. 1, D-7800 Freiburg, Germany

关键词: Zea mays;    Chromosomal protein;    High mobility group protein;    Gene expression;    Transient transformation;   

DOI  :  10.1016/0014-5793(93)80158-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The high mobility group (HMG) proteins represent a class of chromosomal non-histone proteins with an assumed influence on transcription. In this context, the effect of the maize HMGa protein on reporter gene expression was examined. Transient co-transformation experiments in maize protoplasts with plasmid constructs directing the synthesis of the maize HMGa protein and with a luciferase reporter plasmid demonstrated a stimulatory effect of the HMGa protein on the reporter gene expression. Additional experiments with HMGa deletion constructs indicated that the HMG-Box DNA-binding motif is important for the observed effect, while the acidic carboxy-terminal domain of the HMGa protein appears to be dispensable.

【 授权许可】

Unknown