High‐pressure enzyme kinetics

FEBS Letters

Chernyak, B.V.¹  Drachev, V.A.¹  Nikolaeva, N.G.¹  Chernyak, V.Ya.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR

关键词: High pressure;    Kinetics;    Optical cell;    Lactate dehydrogenase;    Deactivation;    Stabilization;    LDH;    lactate dehydrogenase (EC 1.1.1.27);   

DOI  :  10.1016/0014-5793(84)80296-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A newly designed optical cell allows an enzyme reaction to be started under high pressure and makes it possible to begin measurement of the reaction rate after a ‘dead time’ no longer than 1–2 s. This device was used to study the kinetics of lactate dehydrogenase reaction at 1 kbar. At this pressure lactate dehydrogenase from rabbit muscle exhibited a rapid deactivation in the presence of NADH if pyruvate was absent. After addition of pyruvate the reaction was initiated and proceeded at a constant rate, i.e., without loss of enzyme activity. It is suggested that pyruvate markedly increases the association constant of this tetrameric enzyme.

【 授权许可】

Unknown