β‐Hydroxyaspartic acid in vitamin K‐dependent plasma proteins from scorbutic and warfarin‐treated guinea pigs

FEBS Letters

Stenflo, Johan¹  Fernlund, Per¹ 

[1] Department of Clinical Chemistry, University of Lund, Malmö General Hospital, S-214 01 Malmö, Sweden

关键词: Blood coagulation;    γ-Carboxyglutamic acid;    Hydroxylation;    Post-translational modification;    Scurvy;    Vitamin C;    Vitamin K;    Warfarin;   

DOI  :  10.1016/0014-5793(84)80264-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

β-Hydroxyaspartic acid is a rare amino acid, present in all vitamin K-dependent plasma proteins except prothrombin, and is formed by a post-translational hydroxylation of aspartic acid. We have now investigated whether this hydroxylation, like that of proline in collagen, is vitamin C-dependent. The vitamin K-dependent plasma proteins were isolated from normal and scorbutic guinea pig plasma by barium citrate adsorption and the β-hydroxyaspartic acid content was determined. Compared with normal animals, scorbutic animals showed no significant reduction of β-hydroxyaspartic acid content. In warfarin-treated animals there was a decreased content of both β-hydroxyaspartic acid and γ-carboxyglutamic acid in the barium citrate adsorbed fraction. It was concluded that the post-translational hydroxylation of aspartic acid is unlikely to be vitamin C-dependent.

【 授权许可】

Unknown