Rapid identification of Trypanosoma cruzi isolates by ‘dot‐spot’ hybridization

FEBS Letters

Engel, Juan C.¹  Frasch, Alberto C.C.²  Madrid, Raul²  Sanchez, Daniel O.² 

[1] Instituto Nacional de Diagnóstico e Investigación Dr M.F. Chaben, Buenos Aires, Argentina;Catedra de Química Biológica, Facultad de Medicina, Paraguay 2155, 5 Piso, 1121 Capital Federal, Buenos Aires Argentina

关键词: Trypanosoma cruzi;    Kinetoplast DNA;    Minicircle;    Minicircle cloning;    Isolate-specific minicircle;    Isolate identification;    kDNA;    kinetoplast DNA;    SSC;    150 mM NaCl/15 mM sodium citrate;    kb;    kilobasepairs;   

DOI  :  10.1016/0014-5793(84)80223-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of isolate-specific Trypanosoma cruzi minicircles has been shown in the kinetoplast DNA of this parasite. This led to the rapid identification of isolates and clones of trypanosomes by means of ‘dot-spot’ hybridizations with molecularly cloned minicircle probes. Unexpectedly, whole kDNAs were also suitable as probes for this purpose, provided that filters were washed under stringent conditions. This was attributed to the presence of the above-mentioned isolate-specific minicircle sequences. The fact that parasites could be directly spotted onto nitrocellulose filters simplified the rapid routine screening of a large number of samples.

【 授权许可】

Unknown