‘Empty’ minicircles and petB/atpA and psbD/psbE (cytb 559 α) genes in tandem in Amphidinium carterae plastid DNA1

FEBS Letters

Hiller, Roger G.¹ 

[1] Department of Biology, Macquarie University, Macquarie, NSW 2109, Australia

关键词: Minicircle;    Plastid DNA;    Tandem gene organisation;    Dinophycea;    Amphidinium carterae;    PSI;    photosystem 1;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(01)02871-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amphidinium carterae minicircle chloroplast DNA was separated from total DNA by centrifugation through a sucrose/NaCl gradient. Sequences of minicircles with psbA and 23S rRNA contained a common region of 67 bp. Primers designed from this generated numerous polymerase chain reaction products of 1.5–2.6 kb. These contained psaA and psaB as one gene/circle, and petB/atpA and psbD/psbE as two genes/circle. ‘Empty’ minicircles of 1.7–2.5 kb containing no identifiable genes or parts of genes were more abundant than gene-containing circles. From 15 minicircles a minimum common region of 48 bp was identified, with little identity to that from other dinoflagellate minicircles.

【 授权许可】

Unknown