Specific leukotriene formation by purified human eosinophils and neutrophils

FEBS Letters

de Boer, Martin³  Veldink, Gerrit A.¹  Vliegenthart, Johannes F.G.¹  Verhagen, Jan¹  Bruynzeel, Pieter L.B.²  Koedam, Johannes A.¹  Aryan Wassink, G.²  Kreukniet, Johannes²  Terpstra, Gerben K.² 

[1] Department of Bio-Organic Chemistry, State University of Utrecht, Croesestraat 79, NL-3522 AD Utrecht, The Netherlands;Department of Pulmonary Disease, University Hospital, Catharijnesingel 101, NL-3511 GV Utrecht The Netherlands;Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Plesmanlaan 125, NL-1006 AD Amsterdam, The Netherlands

关键词: Leukotriene;    Granulocyte;    Eosinophil;    Neutrophil;    Lipoxygenase;    Asthma;   

DOI  :  10.1016/0014-5793(84)80199-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human granulocytes isolated from peripheral blood have been described to synthesize both LTB₄ and LTC₄ from arachidonic acid. We have observed that the amount of LTC₄ produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium-chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC₄. In contrast, neutrophils produce almost exclusively the chemotactic LTB₄, its ω-hydroxylated metabolite 20-hydroxy-LTB₄ and two non-enzymically formed LTB₄ isomers.

【 授权许可】

Unknown