Evidence for glycosylphosphatidylinositol (GPI)‐anchored eosinophil‐derived neurotoxin (EDN) on human granulocytes

FEBS Letters

Desaint, Corinne³  Schwarz, Ralph T.²  Azzouz, Nahid²  Poussin, Mathilde⁴  Prin, Lionel¹  Gouilleux-Gruart, Valérie³  Debierre-Grockiego, Françoise²  Fuentes, Vincent³  Socié, Gérard⁵ 

[1] Department of Immunology, CHU, F-59037 Lille, France;Institute for Virology, Medical Center for Hygiene, Philipps University, D-35037 Marburg, Germany;Department of Immunology, Faculty of Medicine, F-80036 Amiens, France;Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19139, USA;Service of Bone Marrow Transplantation, Saint Louis Hospital, F-75475 Paris, France

关键词: Neutrophil;    Eosinophil;    Cell surface molecule;    Eosinophil-derived neurotoxin;    Paroxysmal nocturnal hemoglobinuria;    EDN;    eosinophil-derived neurotoxin;    GPI;    glycosylphosphatidylinositol;    PNH;    paroxysmal nocturnal hemoglobinuria;    MNC;    mononuclear cells;   

DOI  :  10.1016/S0014-5793(03)00106-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in specific eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-specific phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was confirmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.

【 授权许可】

Unknown