Characterization of protein kinases in mitotic and meiotic cell extracts

FEBS Letters

Iyer, Anand P.¹  Mastro, Andrea M.¹  Lumley-Sapanski, Katherine¹  Reed, Jon A.¹  Schlegel, Robert A.¹  Halleck, Margaret S.¹ 

[1] Department of Biochemistry, Microbiology, Molecular and Cell Biology, The Pennsylvania State University, University Park, PA 16802, USA

关键词: In situ protein kinase assay;    Mitosis;    Meiosis;    Nuclear membrane breakdown;    Chromosome condensation;    Xenopus oocyte;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(84)80125-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A number of protein kinases have been separated and identified in extracts from mitotic and interphase culture cells and from mature and immature amphibian oocytes using nondenaturing polyacrylamide gel electrophoresis followed by in situ phosphorylation assays. Certain of these protein kinase activities appear to correlate with the biological activity of extracts, assayed by their ability to induce meiotic maturation following injection into Xenopus oocytes. These results are consistent with the notion that protein phosphorylation/dephosphorylation may be integral to the mechanisms of both nuclear membrane breakdown and chromosome condensation, events common and distinctive to mitosis and meiosis.

【 授权许可】

Unknown