Separation of the tryptic fragments of sarcoplasmic reticulum ATPase with high performance liquid chromatography

FEBS Letters

Lüdi, Hans¹  Hasselbach, Wilhelm¹ 

[1] Max-Planck-Institut für Medizinische Forschung, Abteilung Physiologie, Jahnstr. 29, D-6900 Heidelberg 1, FRG

关键词: Sarcoplasmic reticulum;    Ca2+-ATPase;    Tryptic digestion;    Ca2+ binding site;    HPLC;    SDS;    sodium dodecyl sulfate;    Mops;    4-morpholinepropane sulfonic acid;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(84)80827-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The four tryptic fragments of the Ca²⁺- and Mg²⁺-dependent ATPase from sarcoplasmic reticulum were separated on a TSK-G 4000 SW preparative high performance liquid chromatography column in the presence of sodium dodecyl sulfate. Using atomic absorption spectroscopy, the analysis of the protein peaks demonstrated that the calcium binding sites with high affinities are located in the smallest fragment (A₂; 25 kDa).

【 授权许可】

Unknown