Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+‐ATPase mRNA

FEBS Letters

Lompre, Anne-Marie¹  Boheler, Kenneth R.¹  Schwartz, Ketty¹  de la Bastie, Diane¹ 

[1] INSERM U127, Hôpital Lariboisière, 41 Boulevard de la Chapelle, 75010 Paris, France

关键词: Sarcoplasmic reticulum;    ATPase mRNA;    Ca2+-;    Nucleotide sequence;    (Rat heart);    Ca2+-ATPase;    (Ca2+-Mg2+)-dependent ATPase;    SR;    sarcoplasmic reticulum;    ER;    endoplasmic reticulum;    kb;    kilobase;    bp;    base pair;   

DOI  :  10.1016/0014-5793(89)80010-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sarcoplasmic reticulum Ca²⁺-ATPase cDNA clones have been isolated from an adult rat heart cDNA library and the nucleotide sequence of the Ca²⁺-ATPase mRNA determined. The sequence has an open reading frame of 997 codons. It is identical to a cDNA isolated from a rat stomach cDNA library and 90% isologous to the rabbit and human slow/ cardiac cDNAs. Nuclease S₁ mapping analysis indicates that this sequence corresponds to the main Ca²⁺-ATPase mRNA present in heart and in slow skeletal muscle and that it is expressed in various proportions in smooth and non-muscle tissues, together with another isoform which differs from the cardiac form in the sequence of its 3′-end.

【 授权许可】

Unknown