Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2

FEBS Letters

Mauger, Jean-Pierre¹  Claret, Michel¹  Binet, Adrien¹  Berthon, Brigitte¹ 

[1] Laboratoire de Physiologie Cellulaire et des Ensembles Neuronaux, Bât 443, Université Paris-Sud, 91405 Orsay, France

关键词: Hepatocyte;    Quin2;    Cytosolic Ca2+;    Ca2+ influx;    α-Adrenergic agonist;    Vasopressin;    quin2/AM;    quin2 acetoxymethyl tetraester;   

DOI  :  10.1016/0014-5793(84)80824-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytosolic [Ca²⁺] has been measured by using the Ca²⁺-sensitive indicator quin2 in rat liver cells. Optimal loading and hydrolysis have been obtained by equilibrating the cells with 50 μM quin2 acetoxymethyl ester for 150 s. The increase in [Ca²⁺]_i initiated by noradrenaline and vasopressin was reduced but not abolished by removing external Ca²⁺.

【 授权许可】

Unknown