Action of a cytotoxin from Pseudomonas aeruginosaon human leukemic cell lines

FEBS Letters

Lutz, Frieder¹  Sasaki, Terukatsu² 

[1] Institute for Pharmacology and Toxicology, Justus-Liebig University, Frankfurter Strasse 107, D-6300 Giessen, FRG;Department of Biochemistry, Sapporo Medical College, South-1, West-17, Sapporo 060, Japan

关键词: Cytotoxin;    Pseudomonas aeruginosa;    Quin2;    Permeability change;    Human leukemic cell line;    Inositol phosphate;    PtdIns(4;    5)P2 phosphatidylinositol 4;    5-bisphosphate;    PHA;    phytohemagglutinin;   

DOI  :  10.1016/0014-5793(85)80836-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quin2 loaded human leukemic, JURKAT and K562 cells, were exposed to various doses of Pseudomonas aeruginosa cytotoxin. This cytotoxin induced an increase in quin2 fluorescence indicating an increase in the cytoplasmic free Ca²⁺ concentration. The rate of the fluorescence increase and the lag time before the response were dependent on the doses of the cytotoxin. Addition of MnCl₂ to the cytotoxin-treated cells induced a decrease in the quin2 fluorescence at rates dependent on the doses of the cytotoxin. The cytotoxin did not stimulate the inositol lipid turnover in JURKAT cells, which was determined by the accumulation of [³H]inositol phosphates in myo-[2-³H]inositol-prelabeled cells in the presence of LiCl. These results indicate that the cytotoxin increases cell permeability to both Ca²⁺ and Mn²⁺ by direct breakdown of the permeability barrier of the plasma membrane.

【 授权许可】

Unknown