Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic β‐cells

FEBS Letters

Hellman, Bo¹  Rorsman, P.¹  Abrahamsson, H.¹  Gylfe, E.¹ 

[1] Department of Medical Cell Biology, Biomedicum, University of Uppsala, S-751 23 Uppsala, Sweden

关键词: Cytosolic Ca2+;    Quin2;    Insulin secretion;    Glucose;    Voltage-dependent Ca2+-channel;   

DOI  :  10.1016/0014-5793(84)81398-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytosolic Ca²⁺ activity of mouse pancreatic β-cells was studied with the intracellular fluorescent indicator quin2. When the extracellular Ca²⁺ concentration was 1.20 mM, the basal cytosolic Ca²⁺ activity was 162 ± 9 nM. Stimulation with 20 mM glucose increased this Ca²⁺ activity by 40%. In the presence of only 0.20 mM Ca²⁺ or after the addition of the voltage-dependent Ca²⁺-channel blocker D-600, glucose had an opposite and more prompt effect in reducing cytosolic Ca²⁺ by about 15%. It is concluded that an early result of glucose exposure is a lowering of the cytosolic Ca²⁺ activity and that this effect tends to be masked by a subsequent increase of the Ca²⁺ activity due to influx of Ca²⁺ through the voltage-dependent Ca²⁺ channels.

【 授权许可】

Unknown