Purification of a novel pancreatic secretory factor (PSF) and a novel peptide with VIP‐ and secretin‐like properties (helodermin) from Gila monster venom

FEBS Letters

Vandermeers, André¹  Vandermeers-Piret, Marie-Claire¹  Christophe, Jean¹  Waelbroeck, Magali¹  Winand, Jaques¹  Dehaye, Jean-Paul¹  Robberecht, Patrick¹ 

[1] Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Bld. de Waterloo 115, B-1000 Brussels, Belgium

关键词: Gila monster venom;    Pancreatic secretory factor;    Helodermin;    Secretin;    Vasoactive intestinal peptide;    Adenylate cyclase;    VIP;    vasoactive intestinal peptide;    PSF;    pancreatic secretory factor from Gila monster venom;    SDS—PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(84)80094-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A combination of three HPLC procedures applied to the venom of Gila monster (Heloderma suspectum) has led to the purification to homogeneity of two bioactive components: (i) a 17.5 kDa protein, isolated on the basis of its potent secretory effect on dispersed rat pancreatic acini, was accordingly designated PSF (pancreatic secretory factor); (ii) a 5.9-kDa peptide, designated helodermin, was purified on the basis of its ability to stimulate adenylate cyclase in rat pancreatic membranes. PSF was unable to activate adenylate cyclase and, conversely, helodermin was devoid of secretory action.

【 授权许可】

Unknown