An efficient method for labelling antibodies with 111In

FEBS Letters

Buckley, R.G.¹  Searle, F.¹ 

[1] Department of Medical Oncology, Charing Cross Hospital, Fulham Palace Road, London W6 8RF, England

关键词: Isotope labelling;    Indium;    Radionuclide imaging;    DTPA;    diethylenetriaminepentaacetic acid;    a.HCG;    antibody to human chorionic gonadotrophin;    DMSO;    dimethyl sulphoxide;   

DOI  :  10.1016/0014-5793(84)80073-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a new method, rabbit IgG and a monoclonal antibody have been conjugated with the chelating agent DTPA. This was accomplished with reaction conditions that should entail lower antibody damage than existing methods. Gel filtration of the ¹¹¹In-labelled antibody conjugate indicated minimal damage to the antibody and radioimmunoassay showed no significant change in its immunological activity.

【 授权许可】

Unknown