A new reaction useful for chemical cross‐linking between nucleic acids and proteins

FEBS Letters

Negishi, Kazuo¹  Hayatsu, Hikoya¹  Yui, Seiko¹  Nitta, Noriko¹  Kuge, Osamu¹  Tsugawa, Akiko² 

[1] Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700, Japan;Institute of Medical Science, The University of Tokyo, Takanawa, Tokyo 108, Japan

关键词: Chemical cross-linking;    RNA—protein interaction;    Hydrazine—bisulfite;    Cytosine-specific reaction;    Ribosome;    Cam;    N 4-aminocytidine;    imCam;    ethyl-acetimidate-modified N 4-aminocytidine (3-methyl-5;    6-dihydro-s-triazolo[4;    3-c]pyrimidine riboside;   

DOI  :  10.1016/0014-5793(84)80071-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytosine in nucleic acids can be converted into N ⁴-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.

【 授权许可】

Unknown