期刊论文详细信息
FEBS Letters | |
A new reaction useful for chemical cross‐linking between nucleic acids and proteins | |
Negishi, Kazuo1  Hayatsu, Hikoya1  Yui, Seiko1  Nitta, Noriko1  Kuge, Osamu1  Tsugawa, Akiko2  | |
[1] Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700, Japan;Institute of Medical Science, The University of Tokyo, Takanawa, Tokyo 108, Japan | |
关键词: Chemical cross-linking; RNA—protein interaction; Hydrazine—bisulfite; Cytosine-specific reaction; Ribosome; Cam; N 4-aminocytidine; imCam; ethyl-acetimidate-modified N 4-aminocytidine (3-methyl-5; 6-dihydro-s-triazolo[4; 3-c]pyrimidine riboside; | |
DOI : 10.1016/0014-5793(84)80071-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Cytosine in nucleic acids can be converted into N 4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020285134ZK.pdf | 442KB | download |