Characterization of a high‐affinity and specific binding site for Gas6

FEBS Letters

Nakano, Toru¹  Arita, Hitoshi¹  Kishino, Junji¹ 

[1] Discovery Research Laboratories II, Shionogi & Co., Ltd., 5-12-4 Sagisu, Fukuchima-ku, Osaka 553, Japan

关键词: Gas6;    Growth-potentiating factor;    Receptor;    Ca2+ dependence;    Chemical cross-linking;    Gas6;    the protein encoded by growth arrest-specific gene 6;    Gla;    γ-carboxyglutamic acid;    VSMC;    vascular smooth muscle cell;    EGF;    epidermal growth factor;    bFGF;    basic fibroblast growth factor;    DSS;    disuccinimidyl suberate;    PDGF;    platelet-derived growth factor;   

DOI  :  10.1016/0014-5793(96)00394-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have purified a novel growth-potentiating factor and demonstrated that the factor is coded by the gas6 gene. Moreover, we have suggested the presence of a Gas6 receptor on rat vascular smooth muscle cells. In this study, we further analyzed the binding of Gas6 to its receptor. Tissue and cellular distribution of the binding activity of ¹²⁵I-labeled Gas6 showed that the binding site existed in many but a limited range of tissues and cell types. Further characterization of the binding of [¹²¹I]Gas6 using HOS cells demonstrated that the specific binding was dependent on the presence of Ca²⁺. Chemical cross-linking of [¹²⁵I]]Gas6 to HOS cells resulted in the formation of a high-molecular-mass complex, suggesting the presence of a high-molecular-weight receptor.

【 授权许可】

Unknown