Identification of a new 84/82 kDa calmodulin‐binding protein, which also interacts with actin filaments, tubulin and spectrin, as synapsin I

FEBS Letters

Sobue, Kenji¹  Okabe, Toshio¹ 

[1] Department of Neurochemistry and Neuropharmacology, Institute of Higher Nervous Activity, Osaka University Medical School, 4-3-57 Nakanoshima, Kita-ku, Osaka 530, Japan

关键词: Calmodulin-binding protein;    Synapsin I;    Actin filament;    Tubulin;    Spectrin;    Ca2+ dependence;    Calmodulin dependence;    Kinase II;   

DOI  :  10.1016/0014-5793(87)81488-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new 84/82 kDa calmodulin-binding protein, which also interacts with actin filaments, tubulin and spectrin, was purified from the bovine synaptosomal membrane. The binding of calmodulin to this protein was Ca²-dependent, and was inhibited by trifluoperazine, the association constant being calculated to be 2.2 × 10⁶ M⁻¹. Maximally, 1 mol of calmodulin bound to 1 mol of the purified protein. This protein was phosphorylated by both kinase II (Ca²⁺- and calmodulin-dependent kinase) and cyclic AMP-dependent kinase. In addition, antibody against this protein was demonstrated to have an immunological crossreactivity with synapsin I in the synaptosomal membrane.

【 授权许可】

Unknown