RNA—RNA and RNA—protein interactions in 30 S ribosomal subunits Association of 16 S rRNA fragments in the presence of ribosomal proteins

FEBS Letters

Chichkova, N.V.¹  Afonina, E.I.¹  Bogdanov, A.A.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Ribosome structure;    Fragmented 16 S rRNA;    R Nase H hyrolysis;    RNA—RNA interaction;    RNA—protein interaction;    rRNA;    ribosomal RNA;    RNase;    ribonuclease;    RNP;    ribonucleoprotein;    TP30;    total proteins of 30 S subunits;   

DOI  :  10.1016/0014-5793(91)80600-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The E. coli 16 S rRNA with single-site breaks centered at position 777 or 785 was obtained by R Nase H site-specific cleavage of rRNA. Spontaneous dissociation of the cleaved 16 S rRNA into fragments occurred under ‘native’ conditions. The reassociation of the 16 S rRNA fragments was possible only in the presence of ribosomal proteins. The combination of S4 and S16(S17) ribosomal proteins interacting mainly with the 5′-end domain of 16 S rRNA was sufficient for reassociation of the fragments. The 30 S subunits with fragmented RNA at ca. 777 region retained some poly(U)-directed protein synthetic activity.

【 授权许可】

Unknown