Epidermal growth factor: intracellular Ca2+ inhibits its association with pancreatic acini and A431 cells

FEBS Letters

Williams, John A.¹  Logsdon, Craig D.² 

[1] The Departments of Physiology and Medicine, University of California, San Francisco, CA 94143, USA;Cell Biology Research Laboratory, Mount Zion Hospital and Medical Center, PO Box 7921, San Francisco, CA 94120, USA

关键词: EGF;    Calcium;    Receptor;    Pancreas;   

DOI  :  10.1016/0014-5793(83)80312-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The uptake of ¹²⁵I-labeled epidermal growth factor (¹²⁵I-EGF) by mouse pancreatic acini was inhibited (40–50%) by the secretagogue cholecystokinin octapeptide (CCK₈). Analysis of competitive binding data showed that the apparent K _d of EEG binding increased 135% while the binding capacity was only slightly altered (30% increase). That the effect of CCK₈ on acini was mediated by intracellular Ca²⁺ was indicated by the following: (i) Inhibition of ¹²⁵I-EGF binding to acini was dose-dependent and paralleled the known abilities of CCK₈, its analogs, and the cholinergic secretagogue carbachol to induce Ca²⁺ efflux from acini; and (ii) addition of the Ca²⁺ ionophore A23187 also inhibited ¹²⁵I-EGF binding. In addition, EGF association with A431 cells was also inhibited by A23187 in the presence but not the absence of Ca²⁺.

【 授权许可】

Unknown