The CCK receptor on pancreatic plasma membranes: binding characteristics and covalent cross‐linking

FEBS Letters

Sakamoto, Choitsu²  Wong, K.Y.²  Williams, John A.²  Goldfine, Ira D.¹ 

[1] The Departments of Physiology and Medicine, University of California, San Francisco, CA 94143, USA;Cell Biology Research Laboratory, Mount Zion Hospital and Medical Center, PO Box 7921, San Francisco, CA 94120, USA

关键词: Cholectystokinin;    Pancreas;    Receptor;   

DOI  :  10.1016/0014-5793(83)80343-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK₈ > CCK₃₃ > desulphated CCK₈ > CCK₄. Binding of CCK to its receptor was abolished by pretreatment of plasma membranes with trypsin. When [¹²⁵I]CCK was cross-linked to its receptors with disuccinimidyl suberate, and the preparation solubilized and subjected to gel electrophoresis and autoradiography, the hormone was associated with M _r 80000 protein in both the presence and absence of the reducing agent dithiothretol.

【 授权许可】

Unknown