Phosphorylation of a 72‐kDa protein in PDGF‐stimulated cells which forms complex with c‐Crk, c‐Fyn and Eps15

FEBS Letters

Rönnstrand, Lars¹  Heldin, Carl-Henrik¹  Claesson-Welsh, Lena¹  Hansen, Klaus¹ 

[1] Ludwig Institute for Cancer Research, Box 595, Biomedical Center, S-75124 Uppsala, Sweden

关键词: Platelet-derived growth factor;    Receptor;    c-Crk;    c-Fyn;    Eps15;   

DOI  :  10.1016/S0014-5793(97)00495-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ligand-induced activation of the β-receptor for platelet-derived growth factor (PDGF) induces tyrosine phosphorylation of a number of downstream signaling proteins. In the present study, we used two-dimensional gel electrophoresis to characterize the spectrum of proteins phosphorylated in response to PDGF stimulation in porcine aortic endothelial cells expressing PDGF β-receptors. Several previously known substrates for the PDGF β-receptor were identified as well as a novel substrate of 72 kDa. The 72-kDa component could be co-immunoprecipitated in complex with the adaptor protein c-Crk, the non-receptor tyrosine kinase c-Fyn and the signaling molecule Eps15. The results obtained suggests that the 72-kDa protein might play an important role in signaling via the PDGF β-receptor, coupling non-receptor tyrosine kinases of the Src family with c-Crk and Eps15.

【 授权许可】

Unknown