Proteins and polypeptides of envelope membranes from spinach chloroplasts

FEBS Letters

Nguyen, Tan Duc¹  Siegenthaler, Paul-André¹ 

[1] Laboratoire de Physiologie Végétale, Université de Neuchâtel, Chantemerle 20, CH-2000 Neuchâtel, Switzerland

关键词: ATPase;    Chloroplast envelope;    Spinach;    Calmodulin;    Cation activation;   

DOI  :  10.1016/0014-5793(83)80020-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spinach chloroplasts are known to contain calmodulin and to display an envelope-bound ATPase activity. This activity, stimulated by 0.15 mM Ca²⁺ and 5 mM Mg²⁺, is further enhanced by calmodulin. The apparent K _m for ATP was 0.55 mM. The enzyme was especially sensitive to NH₄VO₃, SbCl₃, LaCl₃ and oligomycin. An attempt to isolate the ATPase by calmodulin-Sepharose affinity chromatography was successful. The EGTA-eluted fraction contained 2 proteins out of the 21 proteins separated previously by isoelectric focussing [(1983) Biochim. Acta 722, 226—333] and exhibited an ATPase activity.

【 授权许可】

Unknown