FEBS Letters | |
Proteins and polypeptides of envelope membranes from spinach chloroplasts | |
Nguyen, Tan Duc1  Siegenthaler, Paul-André1  | |
[1] Laboratoire de Physiologie Végétale, Université de Neuchâtel, Chantemerle 20, CH-2000 Neuchâtel, Switzerland | |
关键词: ATPase; Chloroplast envelope; Spinach; Calmodulin; Cation activation; | |
DOI : 10.1016/0014-5793(83)80020-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Spinach chloroplasts are known to contain calmodulin and to display an envelope-bound ATPase activity. This activity, stimulated by 0.15 mM Ca2+ and 5 mM Mg2+, is further enhanced by calmodulin. The apparent K m for ATP was 0.55 mM. The enzyme was especially sensitive to NH4VO3, SbCl3, LaCl3 and oligomycin. An attempt to isolate the ATPase by calmodulin-Sepharose affinity chromatography was successful. The EGTA-eluted fraction contained 2 proteins out of the 21 proteins separated previously by isoelectric focussing [(1983) Biochim. Acta 722, 226—333] and exhibited an ATPase activity.
【 授权许可】
Unknown
【 预 览 】
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