A specific translocator for oxaloacetate transport in chloroplasts

FEBS Letters

Dröscher, L.¹  Flügge, U.I.¹  Heldt, H.W.¹  Hatch, M.D.¹ 

[1] Institut für Biochemie der Pflanze, Universität Göttingen, D-3400 Göttingen, FRG

关键词: Oxaloacetate transport;    Chloroplast envelope;    Malate dehydrogenase;    Photosynthesis;    Spinach;    Maize;   

DOI  :  10.1016/0014-5793(84)81230-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Evidence is provided that oxaloacetate is taken up by chloroplasts from maize and spinach leaves by a high-affinity carrier. Apparent K _m values for oxaloacetate are about 45 and 9 μM, respectively. Uptake was measured by following [4-¹⁴C]oxaloacetate reduction in illuminated chloroplasts. With maize chloroplasts the rates of uptake were sufficient to account for photosynthesis. This carrier had a very low affinity for malate and 2-oxoglutarate. Ratios of the K _i, for malate or 2-oxoglutarate to the K _m for oxaloacetate were about 150 and 50, respectively.

【 授权许可】

Unknown