期刊论文详细信息
FEBS Letters | |
A specific translocator for oxaloacetate transport in chloroplasts | |
Dröscher, L.1  Flügge, U.I.1  Heldt, H.W.1  Hatch, M.D.1  | |
[1] Institut für Biochemie der Pflanze, Universität Göttingen, D-3400 Göttingen, FRG | |
关键词: Oxaloacetate transport; Chloroplast envelope; Malate dehydrogenase; Photosynthesis; Spinach; Maize; | |
DOI : 10.1016/0014-5793(84)81230-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Evidence is provided that oxaloacetate is taken up by chloroplasts from maize and spinach leaves by a high-affinity carrier. Apparent K m values for oxaloacetate are about 45 and 9 μM, respectively. Uptake was measured by following [4-14C]oxaloacetate reduction in illuminated chloroplasts. With maize chloroplasts the rates of uptake were sufficient to account for photosynthesis. This carrier had a very low affinity for malate and 2-oxoglutarate. Ratios of the K i, for malate or 2-oxoglutarate to the K m for oxaloacetate were about 150 and 50, respectively.
【 授权许可】
Unknown
【 预 览 】
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