Subchloroplastic localization of NAD kinase activity: evidence for a Ca2+, calmodulin‐dependent activity at the envelope and for a Ca2+, calmodulin‐independent activity in the stroma of pea chloroplasts

FEBS Letters

Greppin, Hubert¹  Simon, Patrice¹  Marmé, Dieter²  Bonzon, Marc¹ 

[1] Laboratoire de Physiologie Végétale, Université de Genève, 3 place de l'Université, CH-1211 Genève 4, Switzerland;Institut für Biologie III, Universität Freiburg, Schänzlestr. 1, D-7800 Freiburg, FRG

关键词: NAD kinase;    Calmodulin;    Chloroplast envelope;    Pea chloroplast;    CaM;    calmodulin;    Chl;    chlorophyll;    EGTA;    ethylene glycol bis (β-aminoethyl ether)-N;    N.N′;    N′-tetraacetic acid;    flu;    fluphenazine;    GPD;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/0014-5793(84)80152-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chloroplasts were prepared from pea seedlings and tested for NAD kinase activity. More than half of a Ca²⁺, calmodulin-dependent activity and most of a Ca²⁺, calmodulin-independent activity of the homogenate were associated with chloroplasts. The Ca²⁺, calmodulin-dependent activity could be detected by adding Ca²⁺ and calmodulin to the incubation medium containing intact chloroplasts. This activity could not be separated from the chloroplasts by successive washes or by phase partition in aqueous two-polymer phase systems. After chloroplasts fractionation, the Ca²⁺, calmodulin-dependent NAD kinase activity was localized at the envelope, and the Ca²⁺, calmodulin-independent activity was recovered from the stroma. In view of these results and of a previous report [Simon (1982) Plant Cell Rep. 1, 119–122] the occurrence and presumed role of calmodulin in the chloroplast are discussed.

【 授权许可】

Unknown