Two site‐specific endonucleases BinSI and BinSII from Bifidobacterium infantis

FEBS Letters

Kiwaki, Mayumi²  Rak, Bodo¹  Khosaka, Toshiyuki² 

[1] Institute für Biologie III, Universität Freiburg, Schänzlestr. 1, 7800 Freiburg, FRG;Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186, Japan

关键词: Restriction endonuclease;    Isoschizomer;    CC(A T)GG;    GGCGCC;    3′-Terminal extension;    Ligation;   

DOI  :  10.1016/0014-5793(83)80812-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two site-specific endonucleases, BinSI and BinSII, were isolated from Bifidobacterium infantis S76e. BinSI was found to be an isoschizomer of EcoRII, while BinSII was shown to have the same sequence and cutting specificity as BbeI, 5′-GGCGCC-3′. Both BinSII- and BbeI-generated DNA fragments could be ligated with HaeII-generated DNA fragments.

【 授权许可】

Unknown