Isolation and characterization of restriction endonuclease BstYI from Bacillus stearothermophilus Y406

FEBS Letters

Kong, Huimin¹  Chen, Zhongfu¹ 

[1] Institute of Genetics, Fudan University, Shanghai, P.R. China

关键词: Restriction endonuclease;    Isoschizomer;    (Bacillus stearothermophilus);   

DOI  :  10.1016/0014-5793(88)81326-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

BstYI, an isoschizomer of XhoII and MflI, has been purified from Bacillus stearothermophilus Y406. This enzyme recognized 5′…Pu/GATCPy…3′ in DNA and cleaved between Pu and G in this sequence. BstYI can be easily isolated and purified by heparin-agarose column chromatography in a high yield (8000 units BstYI can be obtained per g wet wt of cells).

【 授权许可】

Unknown