Neuropeptide Y in neuroblastoma × glioma hybrid cells

FEBS Letters

MacDermot, J.²  Bloom, S.R.¹  Allen, J.M.¹  Leigh, P.J.²  Yeats, J.C.¹ 

[1] Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Ducane Road, London W12 0HS, England;Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, Ducane Road, London W12 0HS, England

关键词: Neuropeptide Y;    Neuroblastoma cell line;    Dexamethasone;    Nerve growth factor;   

DOI  :  10.1016/0014-5793(83)81162-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

High concentrations of a newly-identified biologically potent peptide, neuropeptide Y, have been demonstrated in 3 related mouse neuroblastoma-derived clonal cell lines, N18TG2 0.35 pmol/mg protein, NG108-15 0.44 pmol/mg protein and NCB-20 0.39 pmol/mg protein. The NG108-15 cell line was chosen for further evaluation. Dexamethasone (10 μM) and nerve growth factor (10 ng/ml) resulted in a 2-fold increase in cellular neuropeptide Y concentrations. The response to dexamethasone was demonstrated to be dose-dependent. Exposure to both agents in combination resulted in a more than additive effect, indicating synergism.

【 授权许可】

Unknown