Specific Expression of Potential Tumour Marker Proteins, Similar to No On or Off Transient A and HIRA-interacting Protein 5, in Mouse N1E-115 Neuroblastoma Cell Line

Cancer Genomics - Proteomics

JOO-HO SHIN¹  EPHREM ENGIDAWORK²  GERT LUBEC¹  JI-EUN OH¹ 

[1] epartment of Paediatrics, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austriaepartment of Paediatrics, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austriaepartment of Paediatrics, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria;epartment of Pharmacology, School of Pharmacy, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopiaepartment of Pharmacology, School of Pharmacy, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopiaepartment of Pharmacology, School of Pharmacy, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia

关键词: Neuroblastoma cell line;    no on or off transient A;    HIRA-interacting protein 5;    proteomics;    biomarker;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Improved cancer detection involving suitable biomarkers with easy applicability is a challenge in our fight against cancer. Biomarkers that provide significant insight into the behaviour of neuroblastoma would greatly aid in identifying patients at risk of disease progression, those whose disease has progressed sub-clinically and those who would benefit from currently available systemic therapies. Materials and Methods: Matrix-assisted laser desorption and ionization-time-of-flight (MALDI-TOF-TOF) is an evolving proteomic technology for improving biomarker discovery, that allows for rapid and sensitive analysis of complex protein mixtures generated from body fluids, cells and/or tissues. MALDI-based profiling identifies unique, differentially-expressed proteins relating to specific cancer-related disease states. A proteomic map of the murine neuroblastoma N1E-115 cell line was generated and MALDI-TOF-TOF utilized in a search for tumour marker candidates. Results: The analytical tool identified and characterized similar to no on or off transient A [fragment] and HIRA-interacting protein 5 proteins that have never been described in any normal or tumour cell lines. Conclusion: These findings suggest that the proteins may serve as candidate markers for screening, staging and drug selection for the management of neuroblastoma, owing to their tumour-specific expression.

【 授权许可】

Unknown