The effect of specific antibodies on oxygen uptake and H+ pumping by cytochrome c oxidase vesicles

FEBS Letters

Freedman, Jo A.¹  Chan, Samuel H.P.¹ 

[1] Biological Research Laboratories, Department of Biology, Syracuse University, Syracuse, NY 13210, USA

关键词: Cytochrome c oxidase;    Reconstituted vesicle;    Proton pumping;    Chemiosmosis;    Antibody;    COV;    cytochrome oxidase vesicles;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    MOPS;    morpholinopropane sulfonic acid;    RCI;    respiratory control index;   

DOI  :  10.1016/0014-5793(83)80785-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies to solubilized cytochrome c oxidse and to subunit III were incubated with liposomal oxidase. In oxygen uptake experiments, the inhibiting effects on RCI of anti-oxidase (primarily anti- subunits II and IV) and anti-III were by different mechanisms: the former, by inhibiting the uncoupled rate; the latter, by stimulating the coupled rate. In experiments with H⁺ translocation, anti-oxidase was without effect, while anti-III was a potent inhibitor of proton pumping. These results are conclusive evidence for redox-linked proton extrusion from the vesicles by the oxidase (and its subunit III).

【 授权许可】

Unknown