An evaluation of the evidence for H+ pumping by reconstituted cytochrome c oxidase in the light of recent criticism

FEBS Letters

Casey, Robert P.¹  Azzi, Angelo¹ 

[1] Medizinisch-chemisches Institut, Universität Bern, Bühlstrasse 28, 3000 Bern 9, Switzerland

关键词: Cytochrome c oxidase;    Proton pumping;    Reconstituted vesicle;    Chemiosmosis;    Energy coupling;    CCCP;    carbonylcyanide m-chlorophenylhydrazone;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    TPP-C1;    tetraphenylphosphonium chloride;    TMPD;    N;    N;    N′;    N′-tetramethyl phenylene diamine;   

DOI  :  10.1016/0014-5793(83)80156-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We review the evidence for H⁺ translocation by reconstituted cytochrome c oxidase; attention is paid to those aspects which we feel most open to criticism. Possible alternative hypotheses are assessed, with regard to experiments carried out to test them directly and with regard to published data. We conclude that, whilst certain aspects of this system are worthy of further clarification, the reported observations are all consistent with proton translocation by reconstituted cytochrome c oxidase; most of these observations provide a positive indication of such an activity.

【 授权许可】

Unknown