Proton‐pump activity of Nitrobacter agilis and Thermus thermophilus cytochrome c oxidases

FEBS Letters

Fukumori, Y.¹  Hon-Nami, K.³  Yanagita, Y.²  Sone, N.²  Yamanaka, T.¹ 

[1] Department of Chemistry, Faculty of Science, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo, 152, Japan;Department of Biochemistry, Jichi Medical School, Minami-kawachi-machi, Tochigi, 329-04, Japan;Mitsubishi-Kasei Institute of Life Sciences, Machida, Tokyo, 194, Japan

关键词: Cytochrome c oxidase;    Proton pumping;    Chemiosmosis;    Nitrobacter agilis;    Thermus thermophilus;    Proteoliposome;    Mops;    4-morpholinopropanesulfonic acid;    FCCP;    carbonyl cyanide p-trifluoromethoxy-phenylhydrazone;   

DOI  :  10.1016/0014-5793(83)80228-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purified cytochrome c oxidases from Nitrobacter agilis and Thermus thermophilus were reconstituted into phospholipid vesicles and their H⁺ pumping activity upon addition of ferrocytochrome c was examined. Thermus cytochrome c oxidase pumped H⁺, while the Nitrobacter enzyme did not. The process of H⁺ movement was successfully simulated.

【 授权许可】

Unknown