Heterogeneity of α2u‐globulin gene products in different translational systems, plasma and urine

FEBS Letters

Vandoren, Gaby¹  Opdenakker, Ghislain³  Verhoeven, Guido¹  Mertens, Bea¹  Volckaert, Guido² 

[1] Laboratorium Experimentele Geneeskunde, 3000 Leuven, Belgium;Laboratorium Genetic Engineering, Rega Instituut, Minderbroedersstraat 10, 3000 Leuven, Belgium;Laboratorium Virologie, 3000 Leuven, Belgium

关键词: Rat liver;    Cell-free translation;    Urinary protein;    Isoelectric focusing;    Recombinant DNA;    Multigene family;   

DOI  :  10.1016/0014-5793(83)80775-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α_2u-Globulin, an androgen dependent rat urinary protein, displays considerable microheterogeneity. To explore whether this microheterogeneity of α_2u-globulin in male rat urine is related to the heterogeneity at the level of the genes encoding this protein, or whether it is due to post-translational processing we studied the α_2u-globulin mRNA translation products in rabbit reticulocyte and Xenopus oocytes. Comparison of the α_2u-globulin species produced in these two heterologous systems with those observed in plasma and urine indicates that the heterogeneity of this protein in urine is mainly due to heterogeneity at the level of the corresponding mRNAs.

【 授权许可】

Unknown