Characteristics of urinary and serum soluble Klotho protein in patients with different degrees of chronic kidney disease

BMC Nephrology

Eiji Kusano²  Makoto Kuro-o¹  Shigeaki Muto²  Yasuhiro Ando²  Kazuhiro Shiizaki¹  Chiharu Ito²  Yoshiyuki Morishita²  Naoko Otani²  Takanori Komada²  Kana Iwazu²  Eri Takeshima²  Tomoyuki Yamazaki²  Akihiko Numata²  Yuko Watanabe²  Hiromichi Yoshizawa²  Tetsu Akimoto² 

[1] Department of Pathology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX, 75390-9072, USA;Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-Shi, TOCHIGI, 329-0498, Japan

关键词: Creatinine;    Urinary protein;    Renal function;    Chronic kidney disease;    Klotho;   

Others  :  1083056
DOI  :  10.1186/1471-2369-13-155

 received in 2012-05-08, accepted in 2012-11-18,  发布年份 2012

【 摘 要 】

Background

Klotho is a single-pass transmembrane protein, which appears to be implicated in aging. The purpose of the present study was to characterize the relationship between the soluble Klotho level and renal function in patients with various degrees of chronic kidney disease (CKD).

Methods

The levels of soluble Klotho in the serum and urine obtained from one hundred thirty-one CKD patients were determined by a sandwich enzyme-linked immunosorbent assay system.

Results

The amount of urinary excreted Klotho during the 24 hr period ranged from 1.6 to 5178 ng/day (median 427 ng/day; interquartile range [IR] 56.8-1293.1), and the serum Klotho concentration ranged from 163.9 to 2123.7 pg/ml (median 759.7 pg/ml; IR 579.5-1069.1). The estimated glomerular filtration rate (eGFR) was significantly correlated with the log-transformed values of the amount of 24 hr urinary excreted Klotho (r = 0.407, p < 0.01) and the serum Klotho levels (r = 0.232, p < 0.01). However, a stepwise multiple regression analysis identified eGFR to be a variable independently associated only with the log-transformed value of the amount of 24-hr urinary excreted Klotho but not with the log-transformed serum Klotho concentration. Despite the strong correlation between random urine protein-to-creatinine ratio and the 24 hr urinary protein excretion (r = 0.834, p < 0.01), a moderate linear association was observed between the log-transformed value of the amount of 24 hr urinary excreted Klotho and that of the urinary Klotho-to-creatinine ratio (Klotho/Cr) in random urine specimens (r = 0.726, p < 0.01).

Conclusions

The amount of urinary Klotho, rather than the serum Klotho levels, should be linked to the magnitude of the functioning nephrons in CKD patients. The use of random urine Klotho/Cr as a surrogate for the amount of 24-hr urinary excreted Klotho needs to be evaluated more carefully.

【 授权许可】

   
2012 Akimoto et al.; licensee BioMed Central Ltd.

【 图 表 】

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