The electron paramagnetic resonance spectra of partially purified cytochrome b 6 f complex from spinach

FEBS Letters

Gerstle, G.C.¹  Salerno, J.C.¹  McGill, J.W.¹ 

[1] Dept. Biology, Rensselaer Polytechnic Institute, Troy, NY 12181, USA

关键词: Cytochrome b 6 f;    EPR spectra;    Semiquinone;    bis-Histidine ligation;    UHDBT;    (Spinach);    UHDBT;    5-undecyl-6-hydroxy-4;    7-dioxobenzothiazole;    EPR;    electron paramagnetic resonance;   

DOI  :  10.1016/0014-5793(83)80767-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In addition to the signals exhibited by cytochrome f and a Rieske-type iron-sulfur cluster, cytochrome b ₆ f preparations exhibit a broad asymmetric peak near g ∼ 3.7 due to cytochrome-b-563 components and a free radical signal which may be a bound semiquinone. Signals near g ∼ 6 and g ∼ 2.9 correspond at least in part to denatured cytochrome b-563; this suggests the possibility of strained bis-histine ligation in the native cytochrome. UHDBT but not antimycin A has a strong specific effect on the spectra of the complex.

【 授权许可】

Unknown