Purification and two‐dimensional crystallization of highly active cytochrome b 6 f complex from spinach

FEBS Letters

Kühlbrandt, Werner¹  Dietrich, Jens¹ 

[1] Max-Planck-Institute of Biophysics, Department of Structural Biology, Heinrich-Hoffmann-Strasse 7, 60528 Frankfurt am Main, Germany

关键词: Cytochrome b 6 f;    Purification;    Two-dimensional crystal;    Spinach;    Hecameg;    6-O-(N-heptylcarbamoyl)-methyl-α-D-glycopyranoside;    DDM;    dodecyl-β-D-maltoside;    PC;    L-α-phosphatidylcholine;    DMSO;    dimethyl sulfoxide;    DOPG;    L-α-dioleoylphosphatidylglycerol;   

DOI  :  10.1016/S0014-5793(99)01609-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purification and two-dimensional crystallization of highly active cytochrome b ₆ f complex from spinach is described. The preparation shows all spectroscopic characteristics of the pure complex. The electron transfer activity of 450±60 electrons per s is the highest in vitro activity reported to date. Using dimethyl sulfoxide (DMSO) as a solvent for the electron donor enhanced the performance and reproducibility of the assay. The high yield and the high activity of the protein make it an ideal candidate for biophysical and structural studies. Preliminary two-dimensional crystallization experiments yielded several different forms of two-dimensional and thin three-dimensional crystals, exhibiting varying degrees of order.

【 授权许可】

Unknown