The transverse membrane orientation of the light‐harvesting and reaction centre polypeptides of Rhodopseudomonas capsulata, investigated by surface iodination

FEBS Letters

Drews, Gerhart¹  Peters, Jürgen D.¹ 

[1] Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Schänzlestr. 1, D-7800 Freiburg, FRG

关键词: Rhodopseudomonas capsulata;    Transverse membrane;    topography;    Bacteriochlorophyll-protein;    Surface iodination;    EDTA;    ethylenediamine tetraacetic acid;    LH;    light-harvesting;    R.;    Rhodopseudomonas;    RC;    reaction centre;    SDS;    sodium dodecylsulphate;   

DOI  :  10.1016/0014-5793(83)81048-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transverse orientation of photosynthetic polypeptides in the membrane of Rhodopseudomonas capsulata was investigated using surface radioiodination of chromatophores and spheroplasts with the reagent 1,3,4,6-tetrachloro-3α, 6α-diphenylglycoluril. Native pigment-protein complexes were isolated by Triton X-100-polyacrylamide gel electrophoresis to identify and distinguish polypeptides not separated by sodium dodecylsulphate-polyacrylamide gel electrophoresis. The two antenna light-harvesting complexes were found to be asymmetrically oriented with polypeptides 14 kDa and 8 kDa of complex B800-850 and 12 kDa of B870 being exposed on either membrane surface. Subunits H and M of the photochemical reaction centre, and probably L, span the membrane.

【 授权许可】

Unknown