Characterization of a new membrane‐bound cytochrome c of Rhodopseudomonas capsulata

FEBS Letters

Drews, Gerhart¹  Hüdig, Hendrik¹ 

[1] Institut für Biologie II, Albert-Ludwigs-Universität, Schänzlestr. 1, D-7800 Freiburg, FRG

关键词: Rhodopseudomonas capsulata;    Cytochrome c;    Cytochrome oxidase;   

DOI  :  10.1016/0014-5793(83)80390-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c ₂ from the same bacterium did not cross react with the membrane-bound cyt. c. The IEP of the membrane-bound cyt. c was found to be pH 8.2 the midpoint potential was 234 ± 11 mV at pH 7.0. This cyt. c binds CO. The native cyt. c is a dimer with an apparent M _r of 25 000 containing 2 mol heme per mol dimer, which is believed to function as an electron donor for the high-potential cytochrome c oxidase.

【 授权许可】

Unknown