Effect of S‐100 protein on microtubule assembly—disassembly

FEBS Letters

Endo, Toyoshi¹  Hidaka, Hiroyoshi¹ 

[1] Department of Pharmacology, Mie University School of Medicine, Tsu 514, Japan

关键词: S-100 protein;    Microtubule;    Assembly;    Disassembly;    Affinity chromatography;    Ca2+;   

DOI  :  10.1016/0014-5793(83)81015-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

S-100 protein was examined for its ability to affect microtubule assembly—disassembly in vitro. Addition of S-100 protein inhibited microtubule assembly in a dose-dependent manner in the presence of Ca²⁺, but this effect was not observed in the presence of 2 mM EGTA. The interaction of microtubule proteins with S-100 protein was also demonstrated by Ca⁺-dependent affinity chromatography on S-100 protein—Sepharose. The proteins bound to the Sepharose column comigrated with tubulin as judged by sodium dodecyl sulfate—polyacrylamide gel electrophoresis. These results might suggest a possible physiological role of S-100 protein in the microtubule assembly—disassembly system.

【 授权许可】

Unknown