Effect of S‐100 protein on assembly of brain microtubule proteins in vitro

FEBS Letters

Donato, Rosario¹ 

[1] Institute of Anatomy, Medical School, Via Del Giochetto, 06100 Perugia, Italy

关键词: S-100 protein;    Microtubule protein;    Calcium;    Potassium;    Assembly;    SDS—PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;    MT;    microtubule;    MES;    2-morpholinoethanesulfonic acid;    EGTA;    ethylene glycol bis(2-aminoethyl-ether)-N;    N′;    N′-tetraacetic acid: A 350;    absorbance at 350 nm;    PC;    phosphocellulose;   

DOI  :  10.1016/0014-5793(83)80778-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

S-100 protein inhibits the assembly of brain microtubule proteins in vitro in the presence of 10 μM free Ca²⁺. The S-100 effect is generally greater on the rate than on the extent of assembly, and even greater as the microtubule protein concentration decreases and the time of preincubation between S-100 and microtubule proteins before GTP addition increases, at a given S-100/tubulin dimer molar ratio. The S-100 effect is greatly enhanced in the presence of physiological concentrations of K⁺ and is completely reversed by EGTA.

【 授权许可】

Unknown