Adenylate cyclase and guanylate cyclase in the excitable ciliary membrane from Paramecium: Separation and regulation

FEBS Letters

Schultz, Joachim E.¹  Gierlich, Doris¹  Klumpp, Susanne¹ 

[1] Pharmazeutisches Institute der Universität, Morgenstelle 8, 7400 Tübingen, FRG

关键词: Adenylate cyclase;    Guanylate cyclase;    Excitable membrane;    Calcium;    Potassium;    Paramecium;   

DOI  :  10.1016/0014-5793(84)80466-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Particulate adenylate cyclase (AC) and guanylate cyclase (GC) activities localized in the ciliary membrane from Paramecium were solubilized by a two-step procedure using the detergents Brij 56 and Lubrol PX. The enzymes remained in the supernatant after a 100 000 × g centrifugation. Upon gel chromatography, AC and GC were almost completely separated proving that each enzyme is a distinct molecular entity. Solubilization of GC was achieved with the calmodulin subunit remaining firmly attached to the catalytic part. Antibodies against calmodulin inhibited the enzyme as did La³⁺ and EGTA. AC activity appeared to be regulated specifically by K⁺, enzyme activity being enhanced up to 100% by 15 mM K⁺. Na⁺ and Li⁺ were inactive.

【 授权许可】

Unknown