Control of nucleation in microtubule self‐assembly

FEBS Letters

Butler, F.M.M.¹  Bayley, P.M.¹  Manser, E.J.¹ 

[1] Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: Microtubule Assembly Nucleation GTP GDP Pyrophosphate;   

DOI  :  10.1016/0014-5793(86)80903-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The inhibition of the rate and amplitude of assembly of microtubule protein at low GTP concentration is shown by measurement of microtubule length distributions to be due to the suppression of microtubule nucleation. This inhibitory effect is enhanced by GDP added before assembly, but can be overcome by a number of molecules such as pyrophosphate or ADP. The selective inhibition of nucleation by GDP in vitro, which occurs in addition to inhibition of elongation, could provide a mechanism for the control of spontaneous microtubule nucleation in vivo.

【 授权许可】

Unknown