ATP synthesis and hydrolysis in submitochondrial particles subjected to an acid—base transition

FEBS Letters

Husain, Iqbal¹  Harris, David A.¹ 

[1] Department of Biochemistry, University of Leeds, Leeds LS2 9JT, England

关键词: Mitochondria;    ATPase inhibitor protein;    Phosphorylation;    ATP hydrolysis;    Acid base transition;    FCCP;    carbonyl cyanide p-trifluoro-methoxyphenylhydrazone;    HEPES;    N-2-hydroxyethyl-piperazine-N'-2-ethane sulphonic acid;   

DOI  :  10.1016/0014-5793(83)80947-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ATP hydrolysis or succinate oxidation by inhibitor-rich submitochondrial particles leads to a 3-fold increase in ATPase activity, with concomitant loss of about 30% of bound inhibitor protein. An acid—base transition causes similar, but smaller, effects (a 30% ATPase increase, and a loss of 8% of the inhibitor). Omitting the electrical component of the gradient completely abolished these effects. The inhibitor protein inhibits ADP phosphorylation induced by an acid—base transition but not by NADH oxidation. This is suggested to reflect the slow movement of the inhibitor protein and the brief period of acid—base jump phosphorylation.

【 授权许可】

Unknown