Protection against lipid peroxidation by a microsomal glutathione‐dependent labile factor

FEBS Letters

Bast, Aalt¹  Haenen, Guido R.M.M.¹ 

[1] Department of Pharmacology and Pharmacotherapy, Faculty of Pharmacy, State University of Utrecht, Catharijnesingel 60, 3511 GH Utrecht, The Netherlands

关键词: Lipid peroxidation;    Glutathione;    Vitamin C;    Ferrous iron;    Liver microsome;   

DOI  :  10.1016/0014-5793(83)80409-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutathione (GSH) protects rat liver microsomes against ascorbic acid (0.2 mM)/ferrous iron (10 μM)-induced lipid peroxidation for some time. The inhibitory effect of GSH is concentration-dependent (0.1–1.0 mM). Our data suggest that GSH acts by preventing initial radical formation rather than via radical scavenging or GSH—peroxidase activity. A labile GSH-dependent factor is involved in the inhibition of microsomal lipid peroxidation by GSH, inasmuch as heating the microsomes abolishes the GSH effect. We found that besides heating, lipid peroxidation also destroys the GSH-dependent factor. Consequently, continuous radical stress will produce lipid peroxidation, despite the presence of GSH. Moreover, a detrimental effect of in vivo-induced lipid peroxidation (CCl₄-treatment) on the GSH-dependent factor was observed. The implications of the present data for the genesis of and the protection against peroxidative damage are discussed.

【 授权许可】

Unknown