Transcribing chromatin is not preferentially enriched with acetylated histones

FEBS Letters

Henmi, Shigeru¹  Yukioka, Munehiko¹  Sasaki, Satoshi¹  Hatayama, Takumi¹  Inoue, Akira¹  Matsuo, Mayumi¹ 

[1] Department of Biochemistry, Osaka City University Medical School, 1-4-54, Asahi-machi, Abeno-ku, Osaka 545, Japan

关键词: Chromatin;    RNA polymerase;    Histone acetylation;    n-Butyrate;    Nuclease digestion;    PMSF;    phenylmethylsulfonyl fluoride;    DTT;    dithiothreitol;    SDS;    sodium dodecyl sulfate;    SnRNA;    small nuclear RNA;   

DOI  :  10.1016/0014-5793(83)80595-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chromatin fragments of the RNA polymerase II-transcriptional complex were purified from the micrococcal nuclease digest of rat liver nuclei in the presence of n-butyrate, a potent histone deacetylase inhibitor. Polyacrylamide gel electrophoretic analysis in Triton acid—urea revealed that the extent of histone acetylation of the complex did not differ markedly from that of the total chromatin.

【 授权许可】

Unknown