n-Butyrate Enhances Induction of Thyroid Hormone- Responsive Nuclear Protein

Endocrine Journal

AKIHIRO SAKURAI¹  SATORU SUZUKI¹  KIYOSHI HASHIZUME¹  KAZUO ICHIKAWA¹  MUTSUHIRO KOBAYASHI¹  TAKAHIDE MIYAMOTO¹  TEIJI TAKEDA¹  YUTAKA NISHII¹ 

[1] Department of Geriatrics, Endocrinology and Metabolism, Shinshu University School of Medicine

关键词: n-Butyrate;    Thyroid hormone action;    Histone acetylation;   

DOI  :  10.1507/endocrj.40.515

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(27)Effects of n-butyrate on nuclear thyroid hormone receptors and on thyroid hormone-responsive nuclear protein were investigated by means of a perfusion system in rat liver. Treatment with 5mM n-butyrate resulted in an increase (150%) in the maximal binding capacity of 3, 5, 3'-L-triiodothyronine (T3) nuclear receptors without altering the affinity of receptor for T3. However, further perfusion for 4h decreased the number of the receptors to the control level. n-Butyrate increased the amount of acetylated histone H4. The ability of nuclear T3 receptors to bind to core histones was diminished by acetylation of the core histones. Thyroid hormone-responsive nuclear protein (n protein) was increased by T3. The induction of the n protein by T3 was augmented by n-butyrate. These results suggested that n-butyrate modulates thyroid hormone-responsive gene expression in rat liver via the increased number of nuclear receptors or changes in the chromatin constitution.

【 授权许可】

Unknown