Polyamines inhibit the yeast histone deacetylase

FEBS Letters

Nelson, Daniel A.¹  Chroneos, Zissis C.¹  Vu, Quang A.¹  Zhang, Dong-er¹ 

[1] Department of Biochemical and Biophysical Sciences, University of Houston, Houston, TX 77004, USA

关键词: Histone deacetylase;    n-Butyrate;    Spermine;    Spermidine;    (Saccharomyces cerevisiae);    Mes;    4-morpholineethanesulfonic acid;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(87)80879-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

n-Butyrate inhibits the histone deacetylase from higher cells, but has little effect on the enzyme activity in Saccharomyces cerevisiae. Spermine and spermidine were therefore tested as potential yeast deacetylase inhibitors and found to inhibit fully the enzyme at 2 and 5 mM, respectively. The utility of these inhibitors was demonstrated by showing that 2 mM spermine substantially increased the incorporation of [³H]acetate into histone in a yeast nuclear acetyltransferase assay.

【 授权许可】

Unknown